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E2636 Sigma

ExtrAvidin® –Alkaline Phosphatase

buffered aqueous solution

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Properties

Related Categories Alkaline Phosphatase Conjugates, Antibodies, Antibody Conjugates, Avidin / Biotin Reagents, Avidin-Biotin Complex Reagents,
form   buffered aqueous solution
application(s)   dot blot: 1:300,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections
  indirect ELISA: 1:60,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Preparation Note

Affinity purified protein

Physical form

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide

Application

ExtrAvidin -Alkaline Phosphatase is suitable for the following applications:
• Dot blot at a dilution of 1:300,000
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100 using human tissues sections
• Indirect ELISA (at a dilution of 1:60,000)
• ELISA
• Enzyme-linked immunosorbent spot (ELISPOT) analysis
• Competitive ELISA

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Immunohistochemistry Procedures

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

Peer-Reviewed Papers
15

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