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  • E2760 - Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

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E2760 Sigma

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone 29.1, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   mouse, pig, human, rat, canine
does not react with   rabbit
application(s)   indirect ELISA: 1:10,000 using extract of the A431 cell line
  western blot: 1:3,000 using extract of the A431 cell line
clone   29.1, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human carcinoma cell line A431, which expresses high levels of EGF receptors.

General description

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (Neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. Monoclonal anti-EGF Receptor detects human, mouse, pig, rat and canine EGF receptor.

Specificity

Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.

Application

Monoclonal Anti-Epidermal Growth Factor Receptor antibody may be used for ELISA at a working antibody dilution of 1:10000 using the extract of A431 cells. For immunoblotting, a dilution of 1:3000 using A431 cell extract is recommended. The antibody may be used to purify EGF receptor, for structural and functional studies of the receptor and for investigation of cell transformation versus oncogenesis. Antibody dilution of 1:50 was used for immunohistochemistry of rat whole ovary sections. The antibody was also used for immunoprecipitation of EGF receptor from extracts of A431 cells.

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