E2761 Sigma


buffered aqueous solution



Related Categories Antibodies, Avidin / Biotin Reagents, Biotin/Avidin, ExtrAvidin Reagents, Molecular Biology,
form   buffered aqueous solution
extent of labeling   3-6 mol fluorochrome per mol protein
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues
  indirect immunofluorescence: 1:200
conjugate   FITC conjugate
shipped in   dry ice
storage temp.   −20°C


Preparation Note

Affinity purified protein

Physical form

Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide


ExtrAvidin®-FITC is suitable for the following applications:
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues.
• Indirect immunofluorescence at a dilution of 1:200
• Immunofluorescence.

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

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Safety Information

NONH for all modes of transport
WGK Germany 
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