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E2761 Sigma

ExtrAvidin®−FITC

buffered aqueous solution

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Properties

Related Categories Antibodies, Avidin / Biotin Reagents, Biotin/Avidin, ExtrAvidin Reagents, Molecular Biology,
form   buffered aqueous solution
extent of labeling   3-6 mol fluorochrome per mol protein
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues
  indirect immunofluorescence: 1:200
conjugate   FITC conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Preparation Note

Affinity purified protein

Physical form

Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide

Application

ExtrAvidin®-FITC is suitable for the following applications:
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues.
• Indirect immunofluorescence at a dilution of 1:200
• Immunofluorescence.

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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