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E2777 Sigma

Monoclonal Anti-c-erbB-2 antibody produced in mouse

~2 mg/mL, clone HER2-96, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-HER-2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   immunoprecipitation: 0.5-1 μg/test using RIPA lysate of cultured CB2 cells (CHO cell line transfected with erbB-2)
  indirect ELISA: suitable
  microarray: suitable
clone   HER2-96, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 185 kDa (complete protein)
  antigen mol wt 97-115 kDa (extracellular ligand-binding domain)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ERBB2(2064)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

purified extracellular domain of human erbB-2 from transfected cells.

General description

ErbB-2 also refers as HER-2 or Neu is an oncogene which encodes a 185kD (p185) transmembrane growth factor orphan receptor. It has a pivotal role in signal transduction, where it can stimulate the signal even in the absence of ligand by forming heterodimers with erbB-1/EGF-R, erbB-3, and erbB-4 and hence can modifies ligand affinity. Monoclonal anti-c-erbB-2 antibody can be used to study the role of erbB-2 and its interaction with erbB family members as well as the cellular molecular mechanisms underlying tumor growth. It can also be used in microarray. Mouse anti-c-erbB-2 antibody reacts specifically with human c-erbB-2 protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal anti-c-erbB-2 antibody can be used in immunoprecipitation (0.5-1 μg/test) using RIPA lysate of cultured CB2 cells (CHO cell line transfected with erbB-2). It can also be used in indirect ELISA.

Features and Benefits

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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References

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