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E2884 Sigma

Esterase from porcine liver

ammonium sulfate suspension, ≥150 units/mg protein (biuret)

Synonym: Carboxyl esterase, Carboxylic-ester hydrolase, PLE

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Description

Application

Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Esterase from porcine liver has been used in a study to assess the reduction of complex coronary atherosclerotic plaque development. Esterase from porcine liver has also been used in a study to investigate the effect of diabetes on bone marrow and circulating porcine endothelial progenitor cells.

The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 8

Biochem/physiol Actions

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.

Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Protocols

Enzymatic Assay of Esterase

To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
Keywords: Addition reactions, Titrations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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N0758 Naphthol AS-D chloroacetate, esterase substrate
N8000 1-Naphthyl butyrate, esterase substrate
B4377 5-Bromo-4-chloro-3-indolyl acetate, esterase substrate
I3500 Indoxyl acetate, ≥95% (GC)
N8130 4-Nitrophenyl acetate, esterase substrate

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