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E2886 Sigma

ExtrAvidin®−Peroxidase

buffered aqueous solution

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Properties

Related Categories Antibodies, Antibody Conjugates, Avidin / Biotin Reagents, Avidin-Biotin Complex Reagents, Biotin/Avidin,
form   buffered aqueous solution
application(s)   direct ELISA: 1:2,000
  dot blot: 1:4,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
conjugate   peroxidase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Preparation Note

Affinity purified protein

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

ExtrAvidin®-Peroxidase is suitable for the following applications:
• Dot blot at a minimum dilution of 1:4,000
• Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
• Direct ELISA at a minimum dilution of 1:2,000
It was used for
• both Immunoprecipitation and Immunobloting
• Ra2I solid-phase binding assays
• Southwestern blotting
• Western blotting
• Immunocytochemistry

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibodies to Peptides and Fusion Tags

Unlike DNA and RNA, which can be targeted with complementary oligonucleotides, protein detection and purification usually relies upon specific antibodies. Although many are available commercially, no...
Leigh Gaskill
BioFiles 2008, 3.6, 14.
Keywords: Biofiles, Immunofluorescence, PAGE, Purification, Sequences, Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Immunohistochemistry Procedures

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

Peer-Reviewed Papers
15

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