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  • E2886 - ExtrAvidin®−Peroxidase

E2886 Sigma


buffered aqueous solution



Related Categories Antibodies, Antibody Conjugates, Avidin / Biotin Reagents, Avidin-Biotin Complex Reagents, Biotin/Avidin,
form   buffered aqueous solution
application(s)   direct ELISA: 1:2,000
  dot blot: 1:4,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
conjugate   peroxidase conjugate
shipped in   wet ice
storage temp.   2-8°C


Preparation Note

Affinity purified protein

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal


ExtrAvidin®-Peroxidase is suitable for the following applications:
• Dot blot at a minimum dilution of 1:4,000
• Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
• Direct ELISA at a minimum dilution of 1:2,000
It was used for
• both Immunoprecipitation and Immunobloting
• Ra2I solid-phase binding assays
• Southwestern blotting
• Western blotting
• Immunocytochemistry

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

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Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibodies to Peptides and Fusion Tags

Unlike DNA and RNA, which can be targeted with complementary oligonucleotides, protein detection and purification usually relies upon specific antibodies. Although many are available commercially, no...
Leigh Gaskill
BioFiles 2008, 3.6, 14.
Keywords: Biofiles, Immunofluorescence, PAGE, Purification, Sequences, Western blot

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Immunohistochemistry Procedures

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