E3011 Sigma


buffered aqueous solution



Related Categories Antibodies, Biochemicals and Reagents, Biotin/Avidin, ExtrAvidin Reagents, Fluorescent Conjugates,
form   buffered aqueous solution
extent of labeling   1-5 mol fluorochrome per mol protein
application(s)   immunohistochemistry: 100 μg/mL using human appendix/tonsil sections and Monoclonal Anti Actin, α-Smooth Muscle as primary antibody
  indirect immunofluorescence: 1:200
conjugate   TRITC conjugate
shipped in   dry ice
storage temp.   −20°C


Preparation Note

Affinity purified protein

Physical form

Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5 and 15 mM sodium azide


ExtrAvidin®-TRITC is suitable for indirect immunofluorescence at a dilution of 1:200.

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and ow background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin® has been conjugated to tetramethylrhodamine isothiocyanate (TRITC) and is used for various techniques.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Antibody Explorer

Explore with Confidence

Amplified Detection
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?