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E3019 Sigma

Esterase from porcine liver

lyophilized powder, ≥15 units/mg solid

Synonym: Carboxyl esterase, Carboxylic-ester hydrolase, PLE

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Description

Application

Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Esterase from porcine liver has been used in a study to assess the effect of 5-aminolaevulinic acid peptide prodrugs on photosensitization for photodynamic therapy. Esterase from porcine liver has also been used in a study to investigate how site-specific atherogenic gene expression correlates with subsequent variable lesion development in coronary and peripheral vasculature.

The enzyme from Sigma has been immobilised in hollow fibre ultrafiltration membrane and used for the asymmetric hydrolysis of a meso-diester. Esterase from Sigma has been used to investigate its effect on the release of methacrylic acid (MAA) and 2-hydroxyethyl methacrylate (HEMA) from adhesives formulated under conditions simulating wet bonding. It has been used to examine the ability of carboxylesterase activity to remove permethrin- and bifenthrin-associated toxicity to Ceriodaphnia dubia and Hyalella azteca in a variety of matrices.

Packaging

3500, 20000, 100000 units in poly bottle

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Physical form

Crude powder containing ≤10% buffer salts

Biochem/physiol Actions

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.

Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Protocols

Enzymatic Assay of Esterase

To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
Keywords: Addition reactions, Titrations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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substrate

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N0758 Naphthol AS-D chloroacetate, esterase substrate
N8000 1-Naphthyl butyrate, esterase substrate
B4377 5-Bromo-4-chloro-3-indolyl acetate, esterase substrate
I3500 Indoxyl acetate, ≥95% (GC)
N8130 4-Nitrophenyl acetate, esterase substrate

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