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  • E3138 - Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

E3138 Sigma

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone F4, ascites fluid

Synonym: Anti-EGFR


Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:2,000
clone   F4, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EGFR(1956)
biological source   mouse
conjugate   unconjugated



synthetically produced residues 985-996 of the human EGF receptor.


This antibody recognizes specifically an intracellular domain of the EGF receptor.


Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse was used for immunoblotting using lysates of mouse liver cells for in vivo protein kinase assay.

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Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.

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