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  • E3152 - Monoclonal Anti-E1 Ubiquitin Activating Enzyme antibody produced in mouse

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E3152 Sigma

Monoclonal Anti-E1 Ubiquitin Activating Enzyme antibody produced in mouse

~2 mg/mL, clone 2G2.3-5, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Protein Degradation, Antibodies to Ubiquitin, Ubiquitin-like and Proteasome Proteins,
species reactivity   mouse, human, rabbit
application(s)   indirect ELISA: suitable
  western blot: 5-10 μg/mL using a whole extract of cultured 293 (human embryonal kidney cells, treated with the proteasome inhibitor MG132
clone   2G2.3-5, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 110 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... UBA1(7317)
mouse ... Uba1(22201)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human and mouse E1

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Monoclonal Anti-E1 Ubiquitin Activating Enzyme antibody produced in mouse is suitable for indirect ELISA and western blotting at a dilution of 5-10μg/mL using a whole extract of cultured 293 (human embryonal kidney cells, treated with the proteasome inhibitor MG132).

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

Ubiquitin-like modifier activating enzyme 1 (UBA1) is an enzyme encoded by UBA1 gene in humans. It is a apical enzyme critical for ubiquitylation-dependent signaling of both DSBs and replication stress in human cells. UBA1 has implications for maintenance of genomic integrity, disease pathogenesis and cancer treatment. Mutation in this gene risks pregnancy.

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