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  • E3526 - Anti-phospho-Elk-1 (pSer383) antibody produced in rabbit

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E3526 Sigma

Anti-phospho-Elk-1 (pSer383) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse, rat, human
application(s)   western blot (chemiluminescent): 1:1,000 using Elk-1 fusion protein phosphorylated with purified Erk2
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... ELK1(2002)
mouse ... Elk1(13712)
rat ... Elk1(314436)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide corresponding to residues around phosphorylated Ser383 of human Elk-1.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

Specificity

The antibody detects Elk-1 protein only when phosphorylated at Ser383.

Application

Anti-phospho-Elk-1 (pSer383) antibody produced in rabbit is suitable for western blotting (chemiluminescent) at a dilution of 1:1,000 using Elk-1 fusion protein phosphorylated with purified Erk2.

Features and Benefits

Antibody Bioguarantee
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Biochem/physiol Actions

ETS domain-containing protein Elk-1 is a protein encoded by the ELK1 gene in humans. It is a member of the Ets family of transcription factors. Elk-1 activation is important for executing its physiologic functions. Its activation is positively associated with ER and with Cyclin D1. Elk1 plays a novel role in controlling cell migration. Elk-1 is involved in a number of important cell processes through the activation of different genes in a number of normal tissues as well as in many malignancies. It is also plays a role in breast neoplasia.

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