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E3528 Sigma

EZview Red HIS-Select® HC Nickel Affinity Gel

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Description

Application

Small-scale affinity capture of fusion proteins with histidine containing tags.

When performing small scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Packaging

1 mL in poly bottle

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Tools for Large Molecule BioAnalysis

Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

EZview™ Red Affinity Gels [VIDEO]

The EZview™ Red Affinity Gels have a vivid red dye that clearly defines the pellet from the supernatant. This enhanced visibility results in less tedious sample processing and greatly reduces the ris...
Keywords: Tagged proteins

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