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E3641 Sigma

Epidermal Growth Factor Receptor human

buffered aqueous glycerol solution, 5,000-30,000 units/mg protein (Lowry)

Synonym: EGFR

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Description

Packaging

500 units in glass bottle

Unit Definition

One unit will catalyze the incorporation of 1 pmol of phosphate from γ-32P-ATP into poly (Glu,Tyr), 4:1 at 30 °C per min.

Physical form

Solution in 50% glycerol, containing 50 mM HEPES, pH 7.6, 150 mM NaCl, 0.1% Triton and 1 mM dithiothreitol

Biochem/physiol Actions

EGF exerts its actions by binding to the EGF receptor (EGFR), a 170 kDa glycoprotein having EGF-activated protein tyrosine kinase activity. Even in the absence of EGF-like ligands, EGFR can participate in cellular responses elicited by several other stimuli. High levels of EGFR are expressed in approximately one third of human epithelial tumors, and in the cancers of the bladder, breast or lung that have poor clinical prognosis, hence the interest in targeting EGFR.

Epidermal growth factor receptor (EGFR) has an essential role in normal developmental processes, and is mutated in multiple cancers. It is involved in the pathogenesis of epidermoid tumors, and thus, has extreme importance as a cancer therapeutic target. Gefitinib (GEF) and erlotinib act as EGFR inhibitors, and induce apoptosis in non-small cell lung cancer (NSCLC) cells. A truncated isoform, called EGFRvIII is linked to multiple cases of head and neck squamous cell carcinoma (HNSCC). In ~40% of glioblastoma multiforme (GBM), this gene is amplified.

General description

Affinity purified from human carcinoma A431 cells.
Epidermal growth factor receptor (EGFR) belongs to a family of cell surface receptors called receptor tyrosine kinases (RTKs), and is also the most studied member of this family. It is made up of an ectodomain (ECD), which contains four subdomains called DI-DIV, a transmembrane region, and a cytoplasmic tyrosine kinase domain (TKD). Binding of the ligand induces conformational changes in the ECD, which leads to the dimerization of EGFR. This results in the activation of the TKD, and the subsequent signal transduction. This receptor has a molecular weight of ∼178kDa.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

EGF/EGFR signaling pathway

Background Epidermal Growth Factor Receptors (EGFR) and ligands     Receptor activation EGFR Signaling     Ras/Raf signaling cascade     Phosphatidylinositol 3-kinase/Akt signaling cascade     Signal...
Keywords: Apoptosis, Cancer, Cell proliferation, Degradations, Diseases, Electron spin resonance, Gene expression, Genetic, Glycosylations, Growth factors, Ligands, Transcription, Transduction

Peer-Reviewed Papers
15

References

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Add to Cart

E2156 Monoclonal Anti-EGF Receptor antibody produced in mouse, clone 225, purified from hybridoma cell culture
HPA001200 Anti-EGFR antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
E1282 Anti-Epidermal Growth Factor Receptor antibody produced in goat, affinity isolated antibody, lyophilized powder
E0532 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone 102618, purified immunoglobulin, lyophilized powder
E2760 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone 29.1, ascites fluid
E3138 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone F4, ascites fluid
E2645 Epidermal Growth Factor Receptor human, lyophilized powder, ≥15,000 units/mg protein (Bradford)

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