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Essential Molecular Biology: A Practical Approach, Volume 2, 2nd ed.

  •  ISBN-10 0-19-963644-3

  •  ISBN-13 978-0-19-963644-0

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publication info   T.A. Brown, ed., Oxford University Press, 2002, 320 pp., soft cover
mfr. no.   Oxford University Press

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General description

In Volume 2 of Essential Molecular Biology, procedures for preparing gene libraries and identifying genes are described, together with methods for studying the structure of a cloned gene and the way it is expressed in the cell.

Table of Contents

1. Strategies for research in molecular biology, T. A. Brown
2. Construction of genomic libraries in lambda and cosmic vectors, Anna-Mariea Frischauf
3. Construction of a cDNA library, Madan K. Bhattacharyya
4. Nucleic acid labeling and detection, C. R. Mundy et al.
5. Immobilization of nucleic acids and hybridization analysis, N. J. Dyson
6. DNA sequencing, T. A. Brown
7. The Polymerace chain reaction, T. A. Brown
8. Protien expression in Escherichia coli, John M. Ward
9. Transcript mapping, Colin P. Smith
Appendices Health hazards and safety procedures
Equipment for molecular biology research
Important Escherichia coli strains
Recipes and general procedures
Restriction endonucleases
DNA and RNA modification enzymes
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