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E3762 Sigma

pFLAG-CMV−5a,b,c Expression Vectors

0.5 mg/mL

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Properties

Related Categories Cloning and Expression, Core Bioreagents, Expression Vectors, For Transient Expression, Life Science Reagents for Transfection,
grade   for molecular biology
assay   0.5 mg/mL
form   buffered aqueous solution
conjugate   FLAG® tagged
shipped in   dry ice
storage temp.   −20°C

Description

Components

• p-FLAG-CMV-5a Expression Vector (20μg)
• p-FLAG-CMV-5b Expression Vector (20 μg)
• p-FLAG-CMV-5c Expression Vector (20 μg)
• pFLAG-CMV-5a-BAP control plasmid (20 μg)

General description

p-FLAG-CMV-5a, p-FLAG-CMV-5b, and p-FLAG-CMV-5c Expression Vectors used for the expression of C-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-5 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS.

Vector Maps and Sequences

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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