pFLAG-CMV™−5a,b,c Expression Vectors
0.5 mg/mL
DOWNLOAD MSDS (PDF)-
MDL number MFCD03100886
Properties
| Related Categories | Cloning and Expression, Core Bioreagents, Expression Vectors, FLAG Protein Expression Systems, For Transient Expression, |
| assay | 0.5 mg/mL |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Other Notes
Browse additional application references in our FLAG® Literature portal.
Packaging
Set includes 20 μg of each vector and pFLAG-CMV-5b-BAP control plasmid.
Application
pFLAG-CMV™-5 a,b,c expression vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG®-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen, such as COS cells. The a,b,c series allows all three reading frames to be available for every restriction site in the multiple cloning site (MCS).
Legal Information
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
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