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E4011 Sigma

ExtrAvidin®−R-Phycoerythrin

buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Biotin/Avidin, ES-EZ,
form   buffered aqueous solution
storage condition   protect from light
extent of labeling   1-4 mol PE per mol ExtrAvidin®
application(s)   flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues
conjugate   phycoerythrin (R-PE) conjugate
storage temp.   2-8°C

Description

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Immunogen

Thymocytes present in C57BL mice.

Packaging

1 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

The ExtrAvidin-R-Phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.

General description

ExtrAvidin, a uniquely modified avidin reagent, combines the high specific activity and sensitivity of avidin with the low background staining of streptavidin. Avidin consists of four identical subunits, each containing a single biotin binding site.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Development of two Multiplex Immunoassays

Depletion of Highly Abundant Protein (HAP) and Moderately Abundant Protein (MAP) from plasma or serum samples can be achieved through Sigma Seppro® Tandem IgY14/ SuperMix columns.
Jiaxin Dong, Chris Melm, Holly Chapman, Farrah Fan,Dian Er Chen, Henry Duewel
Biowire Volume 10 Article 1
Keywords: Enzyme-linked immunosorbent assay, Filtration, High performance liquid chromatography, Immobilization, Indirect ELISA, Mass spectrometry, Proteomics

Related Content

Antibody Explorer - Search 80,000 Antibodies at Sigma-Aldrich

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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