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E4011 Sigma

ExtrAvidin®−R-Phycoerythrin

buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Biotin/Avidin, ES-EZ,
form   buffered aqueous solution
storage condition   protect from light
extent of labeling   1-4 mol PE per mol ExtrAvidin®
application(s)   flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues
conjugate   phycoerythrin (R-PE) conjugate
storage temp.   2-8°C

Description

Features and Benefits

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Immunogen

Thymocytes present in C57BL mice.

Packaging

1 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

The ExtrAvidin-R-Phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.

General description

ExtrAvidin, a uniquely modified avidin reagent, combines the high specific activity and sensitivity of avidin with the low background staining of streptavidin. Avidin consists of four identical subunits, each containing a single biotin binding site.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Development of two Multiplex Immunoassays

Depletion of Highly Abundant Protein (HAP) and Moderately Abundant Protein (MAP) from plasma or serum samples can be achieved through Sigma Seppro® Tandem IgY14/ SuperMix columns.
Jiaxin Dong, Chris Melm, Holly Chapman, Farrah Fan,Dian Er Chen, Henry Duewel
Biowire Volume 10 Article 1
Keywords: Enzyme-linked immunosorbent assay, Filtration, High performance liquid chromatography, Immobilization, Indirect ELISA, Mass spectrometry, Proteomics

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Peer-Reviewed Papers
15

References

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