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E4013 Sigma

Monoclonal Anti-Elastin antibody produced in mouse

clone BA-4, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
species reactivity   human, feline, goat, guinea pig, bovine, sheep, canine, pig
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using sheep arterial tissue
  microarray: suitable
clone   BA-4, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 67.5 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ELN(2006)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

bovine α-elastin.

Specificity

The antibody binds to insoluble elastin, α-elastin, tropoelastin (the soluble non-cross linked precursor of elastin) and to peptide fragments generated by proteolytic digestion of insoluble elastin. In tropoelastin preparations, three immunoreactive bands have been observed at 67.5 kDa, 65 kDa and 62 kDa corresponding to the three elastin isoforms. The antibody is specific for an epitope composed of the repeated sequence in elastin: Val-Gly-Val-Ala-Pro-Gly. This epitope has been shown to be a chemoattractant for fibroblasts and monocytes.

Application

Monoclonal Anti-Elastin antibody produced in mouse is suitable for the following applications:
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:5,000 using sheep arterial tissue
• Microarray
• Immunostaining at a dilution of 1:300.

Features and Benefits

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Biochem/physiol Actions

Elastin is encoded by the ELN gene in humans. It is a polymeric protein in connective tissue that has exceptional properties of extension, elastic recoil to the extracellular matrix and allows many tissues in the body to resume their shape after stretching or contracting. Elastin is a self-assembling, extracellular-matrix protein providing tissue elasticity and is predominantly comprised of cross-linked tropoelastin. It plays an important role in the atherosclerotic process. The conformational disorder is caused due to a constitutive feature of elastin structure and function.

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