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E4127 Sigma

Epidermal Growth Factor from murine submaxillary gland

EGF, suitable for cell culture

Synonym: EGF, Epidermal cell growth factor

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Description

Components

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide present throughout a large number of tissues and body fluids in many mammalian species. EGF is a member of a growth factor family characterized by 6 conserved cysteine motifs that form three disulfide bonds. Human EGF is an identical molecule to ß-urogastrone, a molecule isolated on the basis of its ability to inhibit gastric acid secretion. EGF is structurally homologous to human transforming growth factor-a, and both exert their actions through EGF receptors.

Analysis Note

The biological activity of this product is measured by an ability to stimulate the EGF-responsive mouse BALB/3T3 fibroblast cell line. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Application

This product is used as a mitogen, triggering the cell to commence mitosis, in a variety of cell lines. In tissue cultures, EGF acts to reduce or eliminate the requirement for serum and can be used in conjunction with other media additives and hormone. The working concentration for EGF is generally 0.1-10 ng/mL.

Biochem/physiol Actions

EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, vascular and corneal endothelial cells, bovine granulosa, HeLa cells, SV40-3T3 cells and mammary epithelial cells, rabbit chondrocytes. EGF also plays a biological role in inhibition of gastric acid secretion, support of growth during fetal development and neuromodulation in the central nervous system. Stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins are all cellular metabolic effects of EGF.

Caution

This product should be stored at 2-8°C. After reconstitution, it can be stored at 2-8°C for two weeks, or frozen in aliquots at -20°C for longer periods.

Packaging

0.1 mg in glass bottle

Preparation Note

This product was lyophilized from a solution in 1 mL of 5 mM ammonium acetate at pH 6.5. It should be reconstituted by adding the contents of the vial to a solution containing 0.1-1.0% BSA or 1-10% serum in buffered saline or tissue culture medium.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
KB4518000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

EGF/EGFR signaling pathway

Background Epidermal Growth Factor Receptors (EGFR) and ligands     Receptor activation EGFR Signaling     Ras/Raf signaling cascade     Phosphatidylinositol 3-kinase/Akt signaling cascade     Signal...
Keywords: Apoptosis, Cancer, Cell proliferation, Degradations, Diseases, Electron spin resonance, Gene expression, Genetic, Glycosylations, Growth factors, Ligands, Transcription, Transduction

Epidermal Growth Factors (EGF) and NRG Family

The epidermal growth factor (EGF) family of growth factors is comprised of the normal mammalian gene products of EGF, amphiregulin, betacellulin, heparin-binding EGF (HB-EGF), and transforming growth...
Jennifer Fries
BioFiles 2009, 4.5, 5.
Keywords: Cancer, Growth factors, PAGE, Type

Peer-Reviewed Papers
15

References

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