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E4142 Sigma

ExtrAvidin®−Cy3

buffered aqueous solution

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Properties

Related Categories Antibodies, Avidin / Biotin Reagents, Biotin/Avidin, ExtrAvidin Reagents, Molecular Biology,
form   buffered aqueous solution
extent of labeling   3-9 mol Cy3 per mol protein
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
conjugate   CY3 conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Packaging

1 mL in glass bottle

Preparation Note

Affinity purified protein

Physical form

Solution in 0.01 M phosphate buffered saline, containing 1% bovine serum albumin and 15 mM sodium azide

Application

ExtrAvidin®-Cy3 is suitable for the following applications:
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100
• Immunocytochemistry.
• Used for labeling.

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin and binds biotin with high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin® is covalently conjugated to Cy3 dye and is used for various techniques.

Legal Information

Cy3 is a trademark of GE Healthcare

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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