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  • E4142 - ExtrAvidin®−Cy3

E4142 Sigma


buffered aqueous solution



Related Categories Antibodies, Avidin / Biotin Reagents, Biotin/Avidin, ExtrAvidin Reagents, Molecular Biology,
form   buffered aqueous solution
extent of labeling   3-9 mol Cy3 per mol protein
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
conjugate   CY3 conjugate
shipped in   wet ice
storage temp.   2-8°C



1 mL in glass bottle

Preparation Note

Affinity purified protein

Physical form

Solution in 0.01 M phosphate buffered saline, containing 1% bovine serum albumin and 15 mM sodium azide


ExtrAvidin®-Cy3 is suitable for the following applications:
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100
• Immunocytochemistry.
• Used for labeling.

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin and binds biotin with high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin® is covalently conjugated to Cy3 dye and is used for various techniques.

Legal Information

Cy3 is a trademark of GE Healthcare

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

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NONH for all modes of transport
WGK Germany 
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