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E4274 Sigma

Endotoxin Removal solution

Remove lipopolysaccharides (LPS) from plasmid DNA preparations

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Description

Quantity

1 volume treats 5 volumes of DNA solution (≤1mg/ml)

Application

Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker’s yeast RNA.

Biochem/physiol Actions

The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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