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  • E4644 - Anti-Erythropoietin Receptor antibody produced in goat

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E4644 Sigma

Anti-Erythropoietin Receptor antibody produced in goat

affinity isolated antibody

Synonym: Anti-EPO R, Anti-EPO Receptor

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Hematopoietic Cytokines,
species reactivity   human
application(s)   western blot: 1 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
storage temp.   −20°C
Gene Information   human ... EPOR(2057)
biological source   goat
InChI Key   WOPZJDCVLPFUEY-ZEYSCXRRSA-N
conjugate   unconjugated

Description

Immunogen

recombinant human erythropoietin soluble receptor, expressed in NSO cells.

General description

Erythropoietin Receptor (EPO R) is a protein that belongs to type I cytokine receptor superfamily and is expressed mainly in megakaryocytes, erythoid progenitors, endothelial cells and neurons. Erythropoietin binds to EPO receptor and stimulates Jak2 tyrosine kinase which in turn phosphorylate tyrosine residue of EPO receptor and results in activation of several intracellular pathways (Ras/MAP kinase, phosphatidylinositol 3-kinase, and STAT transcription factors). Anti-Erythropoietin Receptor antibody can be used for analyzing the neurite outgrowth. Goat anti-Erythropoietin Receptor antibody reacts specifically with recombinant human erythropoietin receptor.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Application

Anti-Erythropoietin Receptor can be used in western blotting at a working antibody concentration of 1 μg/ml for detecting human EPO receptor.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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E1407 Monoclonal Anti-Erythropoietin antibody produced in rat, clone 148438, purified immunoglobulin, lyophilized powder

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