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E5134 Sigma

Ethylenediaminetetraacetic acid disodium salt dihydrate

for electrophoresis, for molecular biology, 99.0-101.0% (titration)

Synonym: Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used:
• To prepare TE buffer.
• As an anticoagulant.
• As a constituent of Flow buffer.

Packaging

1 kg in poly bottle

5 kg in poly drum

50, 100, 250, 500 g in poly bottle

General description

Ethylenediaminetetraacetic acid disodium salt dehydrate is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Price and Availability


Interactive Buffer Table

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Respiratory Tract
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
AH4410000
Flash Point(F) 
>212 °F
Flash Point(C) 
>100 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Can solutions of Product E5134, EDTA disodium salt, be autoclaved?
Yes. Please see the product information sheet, which may be accessed though the product detail page: http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/E5134
Is Product E5134, EDTA disodium salt, stable in solution?
Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C, according to the product information sheet. This document may be accessed through the product detail page: http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/E5134
What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?
The optimal concentration is 1.5 mg per mL of blood.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Show more questions
Protocols & Articles

Articles

Ethylenediaminetetraacetic acid disodium salt dihydrate Product Information

Molecular Formula: C10H14N2Na2O8 • 2H2O Molecular Weight: 372.2 CAS Number: 6381-92-6 Melting Point: 248 °C pKa: 2.0, 2.7, 6.2, 10.31
Keywords: Anticoagulants, Biological processes, Melting, Molecular biology

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Peer-Reviewed Papers
15

References

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