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E5144 Sigma

Enterokinase from bovine intestine

powder

Synonym: Enteropeptidase

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Description

Application

Conformation of FLAG peptide removal can be determinied by dot blot assay and SDS-PAGE analysis using nitrocellulose.

Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.

Removes FLAG peptide from N-terminal and Met-N-terminal fusion proteins.

Other Notes

Do not used PVDF since free FLAG peptide will bind to the PVDF membrane.

Packaging

Supplied with optimized enterokinase buffer

General description

Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.

Price and Availability


Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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