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E5906 Sigma

Anti-eIF4E antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CBP, Anti-Eukaryotic intiation factor 4E, Anti-MGC111573, Anti-eIF4E1, Anti-eIF4EL1, Anti-eIF4F

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies for RNA Regulation and Translation, Antibodies to Nuclear Proteins,
species reactivity   human, rat (predicted), mouse
application(s)   immunocytochemistry: 5-10 μg/mL using paraformaldehyde-fixed 3T3 cells
  immunoprecipitation: 2-5 μL using HeLa cell lysates
  western blot: 0.5-1 μg/mL using HeLa cell lysates
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 25 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EIF4E(1977)
mouse ... Eif4e(13684)
rat ... Eif4e(117045)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 21-37 of human eIF4E, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Application

Anti-eIF4E antibody produced in rabbit is suitable for the following applications:
• Immunocytochemistry at a concentration of 5-10μg/mL using paraformaldehyde-fixed 3T3 cells
• Immunoprecipitation at a concentration of 2-5μL using HeLa cell lysates
• Western blotting at a concentration of 0.5-1μg/mL using HeLa cell lysates

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Features and Benefits

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Biochem/physiol Actions

Eukaryotic translation initiation factor 4E is a protein encoded by the EIF4E gene in humans. It is located on human chromosome 20. High level of eIF4E is associated with an increased risk for systemic metastasis in node-positive breast cancer. eIF4E over expression is also associated with a higher incidence of metastasis to multiple sites and acts as a marker for molecular events that increases risk for systemic dissemination. It is also involved in maintaining cell proliferation.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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