|Related Categories||Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,|
|species reactivity||mouse, human, rat|
|western blot: 1:1,000 using human epidermoid carcinoma (A431) cells treated with EGF|
|antibody form||affinity isolated antibody|
|form||buffered aqueous glycerol solution|
|mol wt||antigen mol wt 170 kDa|
|shipped in||wet ice|
human ... EGFR(1956)|
mouse ... Egfr(13649)
synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 845.
The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. 1,2 EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. 3,4 There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis1,4 Interaction of EGF R with c-Src kinase contributes in aggressiveness of human tumors. The phosphorylation of EGF R at Tyr845 and Tyr1101 is mediated by c-Src and helps in the association of c-Src with EGF R5
Anti-phospho-EGFR [pTyr845] specifically recognizes EGFR phosphorylated at tyrosine 845 (170 kDa). The antibody detects human, mouse and rat EGFR [pTyr845].
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/ml BSA (IgG and protease free) and 0.05% sodium azide.
Anti-phospho-EGFR [pTyr845] may be used for immunoblotting at a working dilution of 1:1000 in human A431 cells treated with EGF. The antibody is suitable for immunocytochemistry.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
1. EGF receptor Wells A Int. J. Biochem. Cell Biol. 31, 637-43, (1991)
5. c-Src-mediated phosphorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function Biscardi JS et al J. Biol. Chem. 274, 8335-43, (1999)
Cholesterol depletion inhibits epidermal growth factor receptor transactivation by angiotensin II in vascular smooth muscle cells: role of cholesterol-rich microdomains and focal adhesions in angiotensin II signaling. Ushio-Fukai, M., et al J. Biol. Chem. 276, 48269-48275 , (2001)
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