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E6126 Sigma

Enolase from baker's yeast (S. cerevisiae)

lyophilized powder, ≥50 units/mg protein

Synonym: 2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase

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Properties

Related Categories 4.2.x.x C-O bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, 50-70% modified Warburg-Christian
solubility   15 mM Tris HCl, pH 7.4: soluble1.0 mg/mL, clear
storage temp.   −20°C

Description

Biochem/physiol Actions

Enolase is a metalloenzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is essential for both glycolysis and gluconeogenesis.

Enolase from baker’s yeast is a homodimer containing two bound Mg2+ ions. The molecular weight is 93.069 kDa.The peptide consists of 436 amino acids and contains a single cysteine residue. Two of the active site components include His191 and Arg414. The phosphorylated tyrosine residue present in yeast enolase forms a substrate for phosphorylation by tyrosine protein kinase. Apart from Mg2+, the enzyme can be activated by Zn2+, Mn2+, and Cd2+.

Packaging

500, 2500, 12500 units in poly bottle

Unit Definition

One unit will convert 1.0 μmole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH 7.4 at 25 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Application

Enolase from baker′s yeast has been used in a study to investigate the contribution of the antibodies response induced by a low virulent Candida albicans strain in protection against systemic candidiasis. Enolase from baker′s yeast has also been used in a study to investigate the role of metal ions in catalysis by enolase.

The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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