|Related Categories||Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,|
|species reactivity||rat, human|
|application(s)||western blot: 1:1,000 using EGF-stimulated and non-stimulated NIH3T3 cells expressing human EGFR|
|antibody form||affinity isolated antibody|
|form||buffered aqueous glycerol solution|
|mol wt||antigen mol wt 170 kDa|
|shipped in||wet ice|
|Gene Information||human ... EGFR(1956)|
synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 1068.
The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. 2,3 EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. 4,5 There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis.2,5 Phosphorylation of Tyr1068 occurs by autophosphorylation of EGF R and is required for the binding of Grb2 and activation of ERK1/2 pathway6
Anti-phospho-EGFR [pTyr1068] specifically recognizes human and rat EGFR phosphorylated at tyrosine 1068.
Solution in Dulbecco′s phosphate buffered saline, pH 7.4, with 50% glycerol, 1 mg/ml BSA (IgG, protease free) and 0.05% sodium azide.
Anti-phospho-EGFR [pTyr1068] antibody may be used at a working dilution of 1:1000 for immunoblotting using NIH3T3 cells expressing human EGF R, stimulated with EGF. The antibody has been in detection of EGF R [pTyr1068] in human lymphocytes.1
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
3. Targeting the EGFR signaling pathway in cancer therapy Seshacharyulu P et al Expert Opin. Ther. Targets 16, 15-31, (2010)
Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines. Moro, L., et al. J. Biol. Chem. 277, 9405-9414, (2002)
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