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E6407 Sigma

Anti-eIF2Bε antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Eukaryotic translation initiation factor 2B, ε, Anti-Eukaryotic translation initiation factor 2B, subunit 5, Anti-eIF-2B GDP-GTP exchange factor subunit epsilon

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Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies for RNA Regulation and Translation, Antibodies to Ribosomal Proteins and Translation Factors,
species reactivity   rat, mouse, human
application(s)   indirect immunofluorescence: 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells.
  indirect immunofluorescence: suitable
  western blot: 1:500-1:1,000 using Rat1 or HEK-293T cell lysates.
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~80 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EIF2B5(8893)
mouse ... Eif2b5(224045)
rat ... Eif2b5(192234)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 50-65 of human eIF2ε conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Antigen Background

eIF2Bε encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-eIF2Bε antibody produced in rabbit is suitable for indirect immunofluorescence (at a dilution of 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells) and western blotting at a dilution of 1:500-1:1,000 using Rat1 or HEK-293T cell lysates.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

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Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

Translation initiation factor eIF-2B subunit ε is a protein encoded by the EIF2B5 gene in humans. The eIF2B is composed of five subunits α, β, γ, δ and ε, within which the ε subunit is responsible for catalyzing the guanine exchange reaction. Defect in any of these 5 subunits leads to diseases. Mutation in the subunit of eIF2B is associated with an autosomal recessive leukoencephalopathy called Vanishing white matter (VWM).

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Peer-Reviewed Papers
15

References

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