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E6532 Sigma

Anti-eiF4EBP2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-4E-BP2, Anti-Eukaryotic translation initiation factor 4E-binding protein 2, Anti-eiF4E-binding protein 2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for RNA Regulation and Translation, Antibodies to Ribosomal Proteins and Translation Factors, E-EI,
species reactivity   human, mouse (predicted), rat (predicted)
application(s)   immunoprecipitation: 5-10 μg using lysates of HEK-293T cells over-expressing human eIF4EBP2
  indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human eIF4EBP2
  western blot: 2-4 μg/mL using lysates of HEK-293T cells over-expressing human eIF4EBP2
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~17 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EIF4EBP2(1979)
mouse ... Eif4ebp2(13688)
rat ... Eif4ebp2(361845)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 101-114 of human eIF4EBP2 conjugated to KLH via a cysteine residue. The corresponding sequence differs by one amino acid in mouse and rat.

General description

Eukaryotic translation initiation factor 4E-binding protein 2 (PHASII) is a protein encoded by the EIF4EBP2 gene in humans. Two eukaryotic initiation factor-4E binding proteins (4E-BP1 and 4E-BP2) were found to bind specifically to eIF4E and inhibit its function. 4E-BP2 gene (20kb) exhibits an identical genomic organization to that of 4E-BP1 and is localized to chromosomes 10q21-q22. It is found abundantly in liver and kidney.

Antigen Background

eiF4EBP2 encodes a member of the eukaryotic translation initiation factor 4E binding protein family. The gene products of this family bind eIF4E and inhibit translation initiation. However, insulin and other growth factors can release this inhibition via

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-eiF4EBP2 antibody produced in rabbit is suitable for the following applications:
• Immunoprecipitation at a concentration of 5-10μg using lysates of HEK-293T cells over-expressing human eIF4EBP2
• Indirect immunofluorescence at a concentration of 2.5-5μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human eIF4EBP2
• Western blotting at a concentration of 2-4μg/mL using lysates of HEK-293T cells over-expressing human eIF4EBP2

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Biochem/physiol Actions

The expression of 4E-BP2 acts as a specific repressor of eIF4E function. Its expression is high in double-positive (DP) cells and decreases during maturation, which increases the possibility of a role for 4E-BP2 in repressing eIF4E phosphorylation. 4E-BP2 plays an important role in regulating translation initiation. Its down-regulation is associated with granulocytic maturation and the post-transcriptional regulation is associated with the early action of retinoic acid (RA).

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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WH0001979M4 Monoclonal Anti-EIF4EBP2 antibody produced in mouse, clone 2G8, purified immunoglobulin, buffered aqueous solution

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