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  • E6656 - Anti-eIF4ENIF1 antibody produced in rabbit

E6656 Sigma

Anti-eIF4ENIF1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-4E-T, Anti-Clast4, Anti-Eukaryotic translation initiation factor 4E nuclear import factor 1, Anti-Eukaryotic translation initiation factor 4E transporter, Anti-eIF4E-T



Related Categories Alphabetical Index, Antibodies, Antibodies for RNA Regulation and Translation, Antibodies to Drug Transport Proteins, Antibodies to Ribosomal Proteins and Translation Factors,
species reactivity   human
application(s)   immunoprecipitation: 5-10 μL using HeLa cell lysates
  western blot: 2-4 μg/mL using HeLa cell lysates
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 140 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EIF4ENIF1(56478)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 650-664 of human eIF4ENIF1, conjugated to KLH via an N-terminal added cysteine residue. This sequence is specific to human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.


Anti-eIF4ENIF1 antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μL using HeLa cell lysates and western blotting at a concentration of 2-4μg/mL using HeLa cell lysates.

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Biochem/physiol Actions

Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein encoded by the EIF4ENIF1 gene in humans. It is a nucleocytoplasmic shuttling protein that mediates the import of eIF4E into the nucleus. 4E-transporter (4E-T) is one of several proteins that binds the mRNA 5′cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. It′s interaction with eIF4E is a priming event in inducing messenger ribonucleoprotein rearrangement and transition from translation to decay. Eukaryotic initiation factor 4E plays an important role in controlling cell growth. 4E-T is essential for the assembly of processing bodies and its overexpression triggers the movement of eIF4E into the processing bodies.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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E6781 Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

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