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E6754 Sigma

Epoxy-activated−Sepharose® 6B

lyophilized powder

Synonym: Epoxy-activated-Agarose

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Properties

Related Categories Activated / Functionalized Matrices, Activated/Functionalized Matrices, Affinity Chromatography, Molecular Biology, Protein Chromatography,
form   lyophilized powder
extent of labeling   19-40 μmol per mL
matrix   Sepharose 6B
matrix active group   epoxy
matrix attachment   through epoxy to hydroxyl
matrix spacer   12 atoms (when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.)
swelling   1 g swells to 3.0-5.0 mL
storage temp.   2-8°C

Description

Other Notes

Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Packaging

5, 15 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose and dextran

Application

Epoxy-activated-Sepharose® is used in affinity chromatography, protein chromatography, and activated/functionalized matrices. Epoxy-activated-Sepharose® has been used to obtain a monoclonal antibody against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B as well as to study B meningococcal disease.

Legal Information

Sepharose is a registered trademark of GE Healthcare

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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