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  • E6782 - Anti-ERGIC-53/p58−Cy3 antibody produced in rabbit

E6782 Sigma

Anti-ERGIC-53/p58−Cy3 antibody produced in rabbit

1.5-3.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ERGIC53, Anti-F5F8D, Anti-FMFD1, Anti-LMAN1, Anti-MCFD1, Anti-MR60, Anti-gp58



Related Categories Alphabetical Index, Antibodies, Cell Biology, Cell Signaling and Neuroscience, EO-ER,
species reactivity   human, mouse
application(s)   direct immunofluorescence: 0.5-1.0 μg/mL using human HeLa cells
clone   polyclonal
concentration   1.5-3.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... LMAN1(3998)
mouse ... Lman1(70361)
rat ... Lman1(116666)
biological source   rabbit
conjugate   CY3 conjugate



synthetic peptide corresponding to amino acids 158-170 of rat p58/ERGIC-53/LMAN1, conjugated to KLH. The corresponding sequence is identical in mouse and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-ERGIC-53/p58-Cy3 antibody produced in rabbit is suitable for direct immunofluorescence at a concentration of 0.5-1.0μg/mL using human HeLa cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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Biochem/physiol Actions

Protein ERGIC-53 (also known as ER-Golgi intermediate compartment 53 kDa protein or lectin mannose-binding 1) is a type I integral membrane protein encoded by the LMAN1 gene in humans. ERGIC-53 is a component of ER-Golgi intermediate compartment and is mapped to a YAC and BAC contig containing the critical region for the combined factors V and VIII deficiency gene. It is a dynamic membrane system composed of a number of clusters. ERGIC-53 bypasses the major recycling pathway through Golgi apparatus. It may function as a molecular chaperone during transportation of a specific subset of secreted proteins including coagulation factors V and VIII from ER to Golgi.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

Related Content

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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E1031 Anti-ERGIC-53/p58 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
HPA002320 Anti-LMAN1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

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