|Related Categories||Cloning and Expression, Expression Vectors, For Stable Expression, Mammalian Expression Vectors, Molecular Biology More...|
|grade||for molecular biology|
|shipped in||dry ice|
• pFLAG-CMV™-3 Expression Vector 20 μg (E8770) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pFLAG-CMV™-3-BAP Control Plasmid 20 μg (C3972) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
The pFLAG-CMV™-3 Expression Vector is a 6.3 kb vector used for transient or stable expression in
mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression and secretion of a properly inserted open reading frame as an N-terminal FLAG fusion protein.
The pFLAG-CMV-3 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.
The pFLAG-CMV-3-BAP Control Plasmid is a 7.7 kb derivative of pCMV51 used for transient expression and secretion of N-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The FLAG® epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available from Sigma-Aldrich.
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG-fusion constructs. The preprotrypsin leader sequence precedes the FLAG® sequence and directs secretion of the fusion protein into the culture medium. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418, allowing for selection of stable transfectants.
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
pFLAG-CMV3 is a trademark of Sigma-Aldrich Co. LLC
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Kit includes pFLAG-CMV-3 (E6783) and pFLAG-CMV-4 (E7158)
shuttle vector for transient or stable intracellular expression of C-terminal 3xFLAG
shuttle vector for intracellular transient expression of N-terminal Met-FLAG
shuttle vector for intracellular transient or stable expression of N-terminal Met-FLAG
shuttle vector for intracellular transient expression of C-terminal FLAG
Expression of human MDGA1 increases cell motility and cell-cell adhesion and reduces adhesion to extracellular matrix proteins in MDCK cells. Diaz-Lopez, A., et al. Cancer Microenviron. 4, 23, (2011)
clr-1 encodes a receptor tyrosine phosphatase that negatively regulates an FGF receptor signaling pathway in Caenorhabditis elegans. Kokel M, Borland CZ, DeLong L, et al. Genes Dev. 12(10), 1425-37, (1998)
Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. Gatto G, Dudanova I, Suetterlin P, et al. J. Neurosci. 33(12), 5399-410, (2013)
Signal peptide and denaturing temperature are critical factors for efficient mammalian expression and immunoblotting of cannabinoid receptors. Wang C, Wang Y, Wang M, et al. J. Huazhong Univ. Sci. Technolog. Med. Sci. 32(2), 299-302, (2012)
Adiponectin suppression of high-glucose-induced reactive oxygen species in vascular endothelial cells: evidence for involvement of a cAMP signaling pathway. Ouedraogo R, Wu X, Xu SQ, et al. Diabetes 55(6), 1840-6, (2006)
Molecular cloning and characterization of a novel human beta1,3-glucosyltransferase, which is localized at the endoplasmic reticulum and glucosylates O-linked fucosylglycan on thrombospondin type 1 repeat domain. Sato T, Sato M, Kiyohara K, et al. Glycobiology 16(12), 1194-206, (2006)
Identification and characterization of INCB9471, an allosteric noncompetitive small-molecule antagonist of C-C chemokine receptor 5 with potent inhibitory activity against monocyte migration and HIV-1 infection. Shin N, Solomon K, Zhou N, et al. J. Pharmacol. Exp. Ther. 338(1), 228-39, (2011)
Elucidation of signaling properties of vasopressin receptor-related receptor 1 by using the chimeric receptor approach. Gupte J, Cutler G, Chen JL, et al. Proc. Natl. Acad. Sci. U. S. A. 101(6), 1508-13, (2004)
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|S1797||SOC Medium, For use in transformation|
|L5667||LB Agar Ampicillin-100, Plates|
|E7533||p3XFLAG-CMV™-7.1 Expression Vector, shuttle vector for transient expression of N-terminal 3xFLAG|
|E7283||p3XFLAG-myc-CMV™-26 Expression Vector, shuttle vector for transient or stable intracellular dual tagged expression of N-terminal 3xFLAG and C-term c-myc|
|E7783||p3XFLAG-CMV™-13 Expression Vector, shuttle vector for transient or stable extracellular expression of C-terminal 3xFLAG|
|E7408||p3XFLAG-CMV™-7 Expression Vector, shuttle vector for transient expression of N-terminal Met-3xFLAG|
|E7658||p3XFLAG-CMV™-10 Expression Vector, shuttle vector for transient or stable expression of N-terminal 3xFLAG|
|E7908||p3XFLAG-CMV™-14 Expression Vector, shuttle vector for transient or stable intracellular expression of C-terminal 3xFLAG|
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