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E6907 Sigma

EIF2AK2 (252-end), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: PRK

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Properties

Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
recombinant   expressed in baculovirus infected Sf9 cells
grade   PRECISIO® Kinase
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   51-68 nmol/min·mg
mol wt   mol wt ~64 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... EIF2AK2(5610)

Description

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Biochem/physiol Actions

EIF2AK2 (also known as double-stranded RNA-activated protein kinase) is a protein kinase that has been shown to be involved in HIV/gp120-associated neurodegeneration.EIF2AK2 acts as a critical mediator of gp120 neurotoxicity and is a substrate for a family of protein kinases that respond to various forms of environmental stress. Activation of EIF2AK2 leads to its autophosphorylation and then phosphorylation of its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2. EIF2AK2 plays a critical role in mRNA translation, cell proliferation and apoptosis. A novel cross-talk between the EIF2AKs and p53 has been shown that has implications in cell proliferation and tumorigenesis.

General description

EIF2AK2 (eukaryotic translation initiation factor 2-α kinase 2) is a ubiquitously expressed mammalian Ser/Thr kinase. It is localized to cytoplasm as well as nucleus. Its substrate is α subunit of eukaryotic initiation factor 2 (eIF2α), and it is a constitutively expressed enzyme. This protein is divided into two functional regions- its N-terminal contains two dsRNA binding domains (dsRBDs) and the C-terminal has the kinase domain. In dormant cells, it remains inactivated, but under proper stimuli it either self-activates or is activated by PKR activating protein.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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