|Related Categories||Cloning and Expression, Expression Vectors, For Transient Expression, Mammalian Expression Vectors, Molecular Biology More...|
|grade||for molecular biology|
|shipped in||dry ice|
• pFLAG-CMV™-5.1 Expression Vector 20 μg (E7901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pFLAG-CMV™-5b-BAP Control Plasmid 20 μg (P5225) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
The pFLAG-CMV™-5.1 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector, used for establishing transient intracellular expression of C-terminal FLAG® fusion proteins in mammalian cells.
pFLAG-CMV-5.1 is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.
The pFLAG-CMV-5b-BAP Control Plasmid is a 6.0 kb derivative of the pCMV5 transient expression vector for intracellular expression of C-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus drives transcription of [TM="FLAG′}-fusion constructs. The multiple cloning region of the [TM="pFLAG-CMV′}- 5.1 vector is not preceded by a translational initiation signal. A translational start sequence must be included with the insert DNA. The multiple cloning region of the pFLAG-CMV-5.1 vector is compatible with other CMV vectors.
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
Customers Also Viewed
shuttle vector for intracellular transient expression of N-terminal Met-FLAG
shuttle vector for transient or stable expression of N-terminal FLAG
set of pFLAG-CMV-6a, -6b, and -6c vectors for cytoplasmic expression of N-terminal FLAG
Targeted disruption of Kaposi's sarcoma-associated herpesvirus ORF57 in the viral genome is detrimental for the expression of ORF59, K8α, and K8.1 and the production of infectious virus. Majerciak, V., et al. J. Virol. 81, 1062, (2007)
Add to Cart
|S1797||SOC Medium, For use in transformation|
|L5667||LB Agar Ampicillin-100, Plates|
|E7158||pFLAG-CMV™-4 Expression Vector, shuttle vector for intracellular transient or stable expression of N-terminal Met-FLAG|
|E7033||pFLAG-CMV™-2 Expression Vector, shuttle vector for intracellular transient expression of N-terminal Met-FLAG|
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?