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  • E7028 - Anti-phospho-ERK1 (pThr202/pTyr204) and ERK2 (pThr185/pTyr187) antibody produced in rabbit

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E7028 Sigma

Anti-phospho-ERK1 (pThr202/pTyr204) and ERK2 (pThr185/pTyr187) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-MAPK

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphothreonine (pThr),
species reactivity   human, mouse, rat, chicken
application(s)   immunocytochemistry: suitable
  western blot: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MAPK3(5595)
mouse ... Mapk3(26417)
rat ... Mapk3(50689)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide derived from the region of human ERK1 and 2 that contain threonine 202/185 and tyrosine 204/187 respectively.

General description

Extracellular Signal-Regulated Kinases (ERKs) are members of mitogen-activated protein kinase superfamily (MAPK). MAPK cascade is an evolutionary conserved module that mediates the signaling from various extracellular stimuli to the nucleus. The well-characterized ERK module is activated in response to stimuli such as cytokines, growth factors, osmotic shock, or UV irradiation. ERKs regulate transcription, cell cycle, differentiation, learning, and memory through signal transduction in the cytoplasm and the nucleus. ERK1 and 2 phosphorylate microtubule-associated protein-2 (MAP2), myelin basic protein (MBP), and ELK-1. They may promote entry in the cell cycle. The ERK cascade connects to G proteins through a multitude of distinct signal transduction pathways. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185.

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185. ERK1 and 2 may also undergo autophosphorylation on these residues. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
This antibody recognises endogenous active forms of ERK1/2 (44/42 kDa) in human, mouse, rat and chick embryo.

Physical form

Solution in Dulbecco′s with 50% glycerol, BSA and sodium azide.

Specificity

Rabbit polyclonal anti-phospho-ERK1 (pThr202/pTyr204) and ERK2 (pThr185/pTyr187) antibody recognizes endogenous active forms of ERK1 & 2 (44 kDa and 42 kDa, respectively) in a variety of cell types, including human, mouse, rat and chick embryo.

Application

A working dilution of 1:1000 is recommended for detection of ERK1 (phosphorylated at Thr202/185) and ERK2 (phosphorylated at Tyr204/187) in PC12 cells. The antibody is also suitable for immunocytochemistry and immunohistochemistry in human midbrain sections at a working dilution of 1:500 and 1:2000, respectively.

Rabbit polyclonal anti-phospho-ERK1 (pThr202/pTyr204) and ERK2 (pThr185/pTyr187) antibody may be used in immunoblotting and immunostaining applications. It is used to detect the presence of activated ERK1 and ERK2.

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