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E7032 Sigma

EPHB1 (591-end), active, GST tagged from mouse

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: 9330129L11, AW488255, EPHT2, Elk, Elkh, Hek6, Net

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Properties

Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
recombinant   expressed in baculovirus infected Sf9 cells
grade   PRECISIO® Kinase
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   122-165 nmol/min·mg
mol wt   mol wt ~62 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   mouse ... Ephb1(270190)

Description

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Application

EPHB1 (591-end), active, GST tagged from mouse has been used for in vitro phosphorylation of Caskin1.

Biochem/physiol Actions

EPHB1 (Ephb1 Eph receptor B1) is a member of the Eph family of receptor tyrosine kinases. Ligand-activated EPHB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration. In addition, activated EPHB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. Expression of dominant-negative c-Src significantly reduced EPHB1-dependent ERK1/2 activation and chemotaxis. This receptor is essential for the maintenance of hyperalgesia, and is the predominant receptor for chronic pain in the spinal cord.

General description

EPHB1 (Ephb1 Eph receptor B1) is a glutamatergic synapse forming tyrosine kinase. Mammals contain five EPHB receptors, namely, EPHB1-EPHB4 and EPHB6. Their ligands are transmembrane in nature and are called ephrinB1, -B2 and -B3.

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Price and Availability

Protocols & Articles
Peer-Reviewed Papers
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