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E7033 Sigma

pFLAG-CMV-2 Expression Vector

shuttle vector for intracellular transient expression of N-terminal Met-FLAG

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Properties

Related Categories Cloning and Expression, Core Bioreagents, Expression Vectors, For Transient Expression, Life Science Reagents for Transfection,
grade   for molecular biology
form   buffered aqueous solution
quality   shuttle vector for intracellular transient expression of N-terminal Met-FLAG
conjugate   FLAG® tagged
Peptide tag location   N-terminal
shipped in   dry ice
storage temp.   −20°C

Description

Components

• pFLAG-CMV-2 Expression Vector 20 μg (E7398) is supplied as 0.5 mg/ml in 10 mM Tris-HCl pH 8.0 in 1 mM EDTA.
• pFLAG-CMV-2-BAP Control Plasmid 20 μg (P5100) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

General description

The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG® fusion proteins in mammalian cells.

pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.

The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

Principle

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV2 is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

pSF-FLAG® Vectors from Oxford Genetics

The classic FLAG and 3xFLAG expression vectors have been converted to SnapFast™ Expression Vectors – combining two highly functional systems into one product. Additional information about the SnapFas...
Keywords: Flow cytometry, Gene expression, Immunocytochemistry, Immunoprecipitation, Purification, Western blot

Peer-Reviewed Papers
15

References

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