|Related Categories||Cloning and Expression, Core Bioreagents, Expression Vectors, For Transient Expression, Life Science Reagents for Transfection,|
|grade||for molecular biology|
|quality||shuttle vector for intracellular transient expression of N-terminal Met-FLAG|
|shipped in||dry ice|
• pFLAG-CMV™-2 Expression Vector 20 μg (E7398) is supplied as 0.5 mg/ml in 10 mM Tris-HCl pH 8.0 in 1 mM EDTA.
• pFLAG-CMV™-2-BAP Control Plasmid 20 μg (P5100) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
The pFLAG-CMV™-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG® fusion proteins in mammalian cells.
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigenexpressing host.
The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
pFLAG-CMV2 is a trademark of Sigma-Aldrich Co. LLC
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|S1797||SOC Medium, For use in transformation|
|L5667||LB Agar Ampicillin-100, Plates|
|E7158||pFLAG-CMV™-4 Expression Vector, shuttle vector for intracellular transient or stable expression of N-terminal Met-FLAG|
|E6908||pFLAG-CMV™-5.1 Expression Vector, shuttle vector for intracellular transient expression of C-terminal FLAG|
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