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  • E7153 - Anti-phospho-ERK5 (pThr218/pTyr220) antibody produced in rabbit

E7153 Sigma

Anti-phospho-ERK5 (pThr218/pTyr220) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-BMK1



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphothreonine (pThr),
species reactivity   human
application(s)   western blot: 1:1,000
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MAPK7(5598)
biological source   rabbit
conjugate   unconjugated



Synthetic phosphopeptide derived from the region of human ERK5 that contains threonine 218 and tyrosine 220. This sequence is conserved in human and mouse.

General description

Extracellular Signal-Regulated Kinases (ERKs) are proteins that belong to mitogen-activated protein kinase superfamily (MAPK) and have a pivotal role in cell cycle, differentiation, transcription and learning and memory through signal transduction in the cytoplasm and the nucleus. ERK5 MAP kinase cascade has a crucial functions like- induction of special form of cardiac hypertrophy, enhanced neuronal survival and early steps of muscle cell differentiation. Anti-phospho-ERK5 (pThr218/pTyr220) antibody can be used in immunostaining. Rabbit anti- phospho-ERK5 (pThr218/pTyr220) antibody reacts specifically with ERK5 (88 kD) of human.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide.


Anti-phospho-ERK5 (pThr218/pTyr220) antibody can be used in immunoblotting (diluted 1:1000) using human embryonic kidney cells (HEK-293).

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Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
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WGK Germany  1
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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