• USA Home
  • E7403 - Anti-S1P1, C-Terminal antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
E7403 Sigma

Anti-S1P1, C-Terminal antibody produced in rabbit

~1 mg/mL, fractionated antiserum, buffered aqueous solution

Synonym: Anti-EDG-1, Anti-Endothelial Cell Differentiation Gene-1

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Lipids in Cell Signaling, Primary Antibodies,
species reactivity   human
application(s)   western blot: suitable
clone   polyclonal
concentration   ~1 mg/mL
antibody form   fractionated antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~44 kDa by SDS-PAGE
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... S1PR1(1901)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide (approximately 2.5 kDa) derived from the C-terminal region of the EDG-1/S1P1 receptor.

General description

Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein encoded by the S1PR1 gene in humans. It is a bioactive sphingolipid present at high concentrations in plasma and lymphatic fluid. S1P plays a critical role in regulating lymphocyte trafficking mainly through sphingosine-1-phosphate receptor 1 (S1PR1). It is a blood-borne lysosphingolipid that acts to promote endothelial cell (EC) barrier function. In plasma, it is associated with both high density lipoproteins (HDL) and albumin.

Physical form

Supplied in phosphate buffered saline, pH 7.3, containing 0.08% sodium azide.

Application

Anti-S1P1, C-Terminal antibody produced in rabbit is suitable for western blotting at a working concentration of 5 to 10 μg/ml using RH7777 cells transfected with full-length EDG-1 receptor.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

Sphingosine-1-phosphate (SPP) activates the heterotrimeric guanine nucleotide binding protein (G protein)-coupled orphan receptor EDG-1. EDG-1 is a high-affinity receptor for the bioactive lipid mediator SPP. It binds to SPP with high affinity and specificity. Overexpression of EDG-1 induces exaggerated cell-cell aggregation, enhances expression of cadherins and formation of well-developed adherens junctions. It is essential for lymphocyte egress from secondary lymphoid organs and acts as a validated drug target for the treatment of autoimmune diseases. Activated EDG-1 induces an immediate increase in intracellular calcium and is able to increase S1P1 phosphorylation.

Price and Availability


Antibody Explorer

Explore with Confidence
Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

WGK Germany  -
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?