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E7642 Sigma

Endoglycosidase H from Streptomyces plicatus

recombinant, expressed in E. coli, buffered aqueous solution

Synonym: β-N-Acetylglucosaminidase H

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Description

Unit Definition

One unit will hydrolyze 1.0 μmole of dabsyl-Asn-(GlcNAc)2(Man)5 per min at pH 5.5 at 37 °C.

Physical form

Solution in 0.05 M sodium phosphate, pH 7, containing 25 mM EDTA and preservative

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Endoglycosidases

For some glycoproteins, no cleavage by PNGase F occurs unless the protein is denatured. For others, some or all of the oligosaccharides can be removed from the native protein after extensive incubati...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Buffers, Fucosylation, Type

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Peer-Reviewed Papers
15

References

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