• USA Home
  • E7658 - p3XFLAG-CMV-10 Expression Vector

EMAIL THIS PAGE TO A FRIEND
E7658 Sigma

p3XFLAG-CMV-10 Expression Vector

shuttle vector for transient or stable expression of N-terminal 3xFLAG

Purchase

Properties

grade   for molecular biology
form   buffered aqueous solution
conjugate   3X FLAG tagged
Peptide tag location   N-terminal
shipped in   dry ice
storage temp.   −20°C

Description

Components

p3XFLAG-CMV-10 Expression Vector 20 μg (E4401) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
p3XFLAG-CMV-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

General description

The p3XFLAG-CMV-10 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG® epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. Efficiency of replication is optimal when using an SV40 T antigen expressing host.

The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3X-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloningregion. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.

Vector Maps and Sequences

Principle

The promoter-regulatory region of the human cytomegalovirus3 drives transcription of FLAG®-fusion constructs. The aminoglycoside phosphotransferase II gene (Neo-r) confers resistance to aminoglycosides such as G418 allowing for selection of stable transfectants.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.

p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

pSF-FLAG® Vectors from Oxford Genetics

The classic FLAG and 3xFLAG expression vectors have been converted to SnapFast™ Expression Vectors – combining two highly functional systems into one product. Additional information about the SnapFas...
Keywords: Flow cytometry, Gene expression, Immunocytochemistry, Immunoprecipitation, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

S1797 SOC Medium, For use in transformation
L5667 LB Agar Ampicillin-100, Plates, pre-poured agar plates with 100 μg/ml ampicillin
E7533 p3XFLAG-CMV-7.1 Expression Vector, shuttle vector for transient expression of N-terminal 3xFLAG
E7283 p3XFLAG-myc-CMV-26 Expression Vector, shuttle vector for transient or stable intracellular dual tagged expression of N-terminal 3xFLAG and C-term c-myc
E7783 p3XFLAG-CMV-13 Expression Vector, shuttle vector for transient or stable extracellular expression of C-terminal 3xFLAG
E7408 p3XFLAG-CMV-7 Expression Vector, shuttle vector for transient expression of N-terminal Met-3xFLAG
E6783 pFLAG-CMV-3 Expression Vector, shuttle vector for transient or stable expression of N-terminal FLAG
E7908 p3XFLAG-CMV-14 Expression Vector, shuttle vector for transient or stable intracellular expression of C-terminal 3xFLAG
OGS10 PSF-CMV-KAN - KANAMYCIN RESISTANCE PLASMID, plasmid vector for molecular cloning
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?