p3XFLAG-CMV™-13 Expression Vector
shuttle vector for transient or stable extracellular expression of C-terminal 3xFLAG
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Properties
| Related Categories | Cloning and Expression, FLAG Protein Expression Systems, Mammalian FLAG Expression Vectors, Molecular Biology |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Other Notes
Browse additional application references in our FLAG® Literature portal.
Application
p3XFLAG-CMV™-13 is an expression vector that can be used as a shuttle vector for Escherichia coli and mammalian cells. It can used for transient or stable expression and extracellular secretion of C-terminal 3XFLAG® fusion proteins in mammalian cells. Transcription of FLAG®-fusion constructs is driven by the promoter-regulatory region of human cytomegalovirus. Stable transfectants can be selected for resistance to neomycin (G418 selection). The vector contains both bacterial and SV40 origins of replication, which results in optimal replication in host cells expressing the SV40 large T antigen, such as COS cells. The use of the 3XFLAG® tag results in increased sensitivity of detection using ANTI-FLAG® M2 antibody detection systems.
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
Kit component only
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Description |
Product # |
Add to Cart |
|---|---|---|
| p3XFLAG-CMV™-13 Expression Vector | ||
| Control Vector, p3xFLAG-CMV™-7-BAP |
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