p3XFLAG-CMV™-14 Expression Vector
shuttle vector for transient or stable intracellular expression of C-terminal 3xFLAG
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Properties
| Related Categories | Bacterial Expression Vectors, Cloning and Expression, Expression Vectors, For Stable Expression, Mammalian Expression Vectors, |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
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Application
p3XFLAG-CMV™-14 can be used as a shuttle vector for Escherichia coli and mammalian cells. It is used for transient or stable intracellular expression of C-terminal 3XFLAG® fusion proteins in mammalian cells. Transcription of FLAG®-fusion constructs is driven by the promoter-regulatory region of human cytomegalovirus. Stable transfectants can be derived by selection for resistance to neomycin (G418 selection). The vector contains both bacterial and SV40 origins of replication, which results in optimal replication in host cells expressing the SV40 large T antigen, such as COS cells. The use of the 3XFLAG® tag results in increased sensitivity of detection using ANTI-FLAG® M2 antibody detection systems.
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
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|
Description |
Product # |
Add to Cart |
|---|---|---|
| p3XFLAG-CMV™-14 Expression Vector | ||
| Control Vector, p3xFLAG-CMV™-7-BAP |
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