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E8140 Sigma

Elastase from human leukocytes

lyophilized powder, ≥50 units/mg protein (Bradford)

Synonym: Lysosomal elastase



Biochem/physiol Actions

Unlike pancreatic elastase the leukocyte enzyme has a preferential cleavage for the carboxyl side of valine, but will also cleave to a lesser extent after alanine. Natural substrates include elastin, cartilage proteoglycans, collagen types I, II, II and IV, and fibronectin.

Unit Definition

One unit will release one nanomole of p-nitrophenol per sec from BOC-L-alanine p-nitrophenyl ester at pH 6.5 at 37 °C.

Physical form

Lyophilized from 0.05 M sodium acetate (pH 5.5) and 0.6 M NaCl


Elastase from Sigma has been used to digest fibronectin. The results were compared with fibronectin digestion by crude human leukocyte homogenateto examine the presence of fibronectin peptides in saliva of patients with Sjögren′s syndrome. It has also been used as a reference to determine the elastase activity in cell lysates. This study examined the effect of all-trans retinoic acid on procoagulant and fibrinolytic activities of cultured blast cells. These blast cells were from patients with acute promyelocytic leukemia.

Elastase from human leukocytes has been used in a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal models.

Physical properties

Leukocyte elastase is a 29 kDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds. It contains two or thee N-linked glycans of variable composition which account for its three major isoforms.
Isoelectric point: pI = 8.77 - 9.55

Price and Availability

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange
Keywords: Chromatography, High performance liquid chromatography

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


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