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E8152 Sigma

EphA3/Fc Chimera from mouse

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym: Cek4, Hek4, Hek, Mek4, Tyro4

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Properties

Related Categories Angiogenesis, Application Index, Biochemicals and Reagents, Cancer Research, Cell Biology,
recombinant   expressed in NSO cells
assay   >90% (SDS-PAGE)
form   lyophilized powder
mol wt   monomer calculated mol wt 86 kDa
  mol wt ~110 kDa by SDS-PAGE (reducing)
impurities   endotoxin, tested
storage temp.   −20°C
Gene Information   mouse ... Epha3(13837)

Description

Analysis Note

The biological activity is measured by its ability to bind recombinant human ephrin-A5/Fc in an ELISA.

Biochem/physiol Actions

Member of the Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4, ephrin-A5, and ephrin-B1; involved in pattern formation and morphogenesis.

EPHA3 binds the ephrin-A ligand and has diverse cellular function. Analysis of human colorectal, breast, lung and pancreatic cancer samples shows somatic mutations in the EPHA3 gene. EPHA3 gene expression can be regulated by CD28 and IGF-1 in Jurkat cells and expression of EPHA3 is associated with adherence and motility of malignant T cells. It maintains the less differentiated state of cancer cells, by activating MAPK (mitogen-activated protein kinase) pathway. It is over-expressed in glioblastoma (GBM) cells, especially during the initial stages. It also higher expression levels in aggressive mesenchymal subtype of GBM. In hepatocellular carcinoma (HCC), it predicts increased invasiveness and poor patient prognosis.

Other Notes

Extracellular domain of mouse EphA3 (amino acids 1-541) fused by means of a polypeptide linker to the Fc region of human IgG1 that is histidine tagged at the C-terminus.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

General description

EPHA3 is a receptor tyrosine kinase (RTK) belonging to the largest subgroup of RTKs called ephrin group of receptors. It has a high level of expression from placental stage through the whole developmental process. It is a type I transmembrane protein, and has a membrane-bound ligand. It is both an oncogene and a tumor-suppressor gene. This protein resides in both the cytosol and the plasma membrane.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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