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E8777 Sigma

EphA8/Fc Chimera from mouse

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym: Eek, Hek3

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
recombinant   expressed in NSO cells
assay   >90% (SDS-PAGE)
form   lyophilized powder
mol wt   monomer calculated mol wt 84.2 kDa
  mol wt 100-120 kDa by SDS-PAGE (reducing)
impurities   endotoxin, tested
storage temp.   −20°C
Gene Information   mouse ... Epha8(13842)

Description

Analysis Note

The biological activity is measured by its ability to bind recombinant human ephrin-A5/Fc in an ELISA assay.

Biochem/physiol Actions

Member of the Eph receptor tyrosine kinase family shown to bind ephrin-A2, ephrin-A3, and ephrin-A5; involved in pattern formation and morphogenesis.

EphA8 (ephrin receptor A8) interacts with the phosphotyrosine-binding (PTB) domain of protein of Anks family. This in turn, modulates the signaling cascade of EphA8. It also binds to the SAM domain of Anks proteins, such as Odin, which then regulate the ubiquitination and degradation of EphA8. Meis and Pbx, which are TALE homeobox proteins, act as regulatory transcription factors for EphA8 protein, during mesencephalon development. The juxtamembrane segment of this protein is essential in regulating cell adhesion and migration. It co-localizes with Odin protein at the neurite tips, where it functions in axon guidance.

Other Notes

Extracellular domain of mouse EphA8 (amino acids 1-540) fused by means of a polypeptide linker to the Fc region of human IgG1 that is C-terminal histidine-tagged at the C-terminus.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.

General description

EphA8 (ephrin receptor A8) is a receptor tyrosine kinase (RTK), which belongs to a 16 member family of ephrin receptors. This family is divided into two classes A and B. EphA8 belongs to EphA group, which contains 10 members. The ligands to this group of receptors are glycosylphosphatidylinositol (GPI)-anchored named ephrin-A1-A6. The members of this family have an ephrin-binding domain in its N-terminal globular domain, succeeded by a linker region rich in cysteine and two fibronectin type III repeats in the exoplasmic domain. Its cytoplasmic domain consists of a juxtamembrane region, a conserved tyrosine kinase domain, an α-helical C-terminal domain, and a PDZ domain-binding domain.

Price and Availability


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Biomedical Applications
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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