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E8781 Sigma

Anti-EDEM3 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ER degradation enhancer, mannosidase alpha-like 3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
species reactivity   mouse, rat, human
application(s)   immunoprecipitation: 1-2 μL using mouse 3T3 cell lysates
  western blot: 1:1,000-1:2,000 using whole extract of human HEK-293T cells
  western blot: 1:500-1:1,000 using whole extrct of rat PC12 cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~120 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EDEM3(80267)
mouse ... Edem3(66967)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 869-882 of human EDEM3, conjugated to KLH via an N-trerminal cysteine residue. The corresponding sequence is identical in mouse and rat EDEM3.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-EDEM3 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 1-2μL using mouse 3T3 cell lysates and western blotting (at a dilution of 1:1,000-1:2,000 using whole extract of human HEK-293T cells and at a dilution of 1:500-1:1,000 using whole extrct of rat PC12 cells).

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Biochem/physiol Actions

ER degradation-enhancing α-mannosidase-like 3 is an enzyme encoded by the EDEM3 gene in humans and consists of 931 amino acids. It consists of motifs of Class I α-mannosidases (glycosyl hydrolase family 47) in its N-terminal domain and a protease-associated motif in its C-terminal region. EDEM3 has α1,2-mannosidase activity. EDEM3 is also aasociated with folding-incompetent proteins carrying N-glycans that are extracted from futile folding cycles in the calnexin chaperone system.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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E9906 Anti-EDEM2 (N-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
E1782 Anti-EDEM2 (C-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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